Doctor of Philosophy

dissertationMultiple-input and multiple-output (MIMO) technique has emerged as a key feature for future generations of wireless communication systems. It increases the channel capacity proportionate to the minimum number of transmit and receive antennas. This dissertation addresses the receiver design for high-rate MIMO communications in at fading environments. The emphasis of the thesis is on the cases where channel state information (CSI) is not available and thus, clever channel estimation algorithms have to be developed to bene t from the maximum available channel capacity. The thesis makes four distinct novel contributions. First, we note that the conventional MCMC-MIMO detector presented in the prior work may deteriorate as SNR increases. We suggest and show through computer simulations that this problem to a great extent can be solved by initializing the MCMC detector with regulated states which are found through linear detectors. We also introduce the novel concept of staged-MCMC in a turbo receiver, where we start the detection process at a lower complexity and increase complexity only if the data could not be correctly detected in the present stage of data detection. Second, we note that in high-rate MIMO communications, joint data detection and channel estimation poses new challenges when a turbo loop is used to improve the quality of the estimated channel and the detected data. Erroneous detected data may propagate in the turbo loop and, thus, degrade the performance of the receiver signi cantly. This is referred to as error propagation. We propose a novel receiver that decorrelates channel estimation and the detected data to avoid the detrimental e ect of error propagation. Third, the dissertation studies joint channel estimation and MIMO detection over a continuously time-varying channel and proposes a new dual-layer channel estimator to overcome the complexity of optimal channel estimators. The proposed dual-layer channel estimator reduces the complexity of the MIMO detector with optimal channel estimator by an order of magnitude at a cost of a negligible performance degradation, on the order of 0.1 to 0.2 dB. The fourth contribution of this dissertation is to note that the Wiener ltering techniques that are discussed in this dissertation and elsewhere in the literature assume that channel (time-varying) statistics are available. We propose a new method that estimates such statistics using the coarse channel estimates obtained through pilot symbols. The dissertation also makes an additional contribution revealing di erences between the MCMC-MIMO and LMMSE-MIMO detectors. We nd that under the realistic condition where CSI has to be estimated, hence the available channel estimate will be noisy, the MCMC-MIMO detector outperforms the LMMSE-MIMO detector with a signi cant margin

Screening of differentially expressed proteins from syncytiotrophoblast for severe early-onset preeclampsia in women with gestational diabetes mellitus using tandem mass tag quantitative proteomics

Abstract Background Previous studies have revealed that women with gestational diabetes mellitus (GDM) have an increased risk of developing preeclampsia (PE). The possible reason is the abnormal lipid metabolism caused by GDM that leads to dysfunction of vascular endothelial cells and atherosclerosis, resulting in the onset of PE. However, studies focusing on the pathogenesis of PE in syncytiotrophoblast of GDM patients are lacking. This study aimed to compare differentially expressed proteins from syncytiotrophoblast between women with GDM and women with GDM with subsequently developed PE. Methods Syncytiotrophoblast samples were obtained from pregnant women immediately after delivery. To explore the protein expression changes of syncytiotrophoblast that might explain the pathogenesis of PE in women with GDM, quantitative proteomics was performed using tandem mass tag (TMT) isobaric tags and liquid chromatography-tandem mass spectrometry. Bioinformatics analysis was performed to enrich the biological processes that these differentially expressed proteins were involved in. Results A total of 28,234 unique peptides and 4140 proteins were identified in all samples. Among them, 23 differentially expressed proteins were identified between patients with GDM and patients with GDM with subsequently developed PE. Therein, 11 proteins were upregulated and 12 proteins were downregulated. Two relative proteins (FLT1 and PABPC4) were independently verified using immunoblotting analysis. Bioinformatic results indicated that the onset of PE in patients with GDM is a multifactorial disorder, involving factors such as apoptosis, transcriptional misregulation, oxidative stress, lipid metabolism, cell infiltration and migration, and angiogenesis. Conclusion These results indicated that the inadequacy of endometrium infiltration, angiogenic disorder, and oxidative stress in syncytiotrophoblast are more likely to occur in patients with GDM and may be the potential mechanisms leading to such patients secondarily developing severe early-onset PE

Rational Designed Bipolar, Conjugated Polymer-DNA Composite Beacon for the Sensitive Detection of Proteins and Ions

Nature owns remarkable capabilities in sensing target molecules, while the artificial biosensor lags far behind nature. Inspired by nature, we devise a new sensing platform that can specifically bind the molecules and synchronously initiate a specific signal response. We rationally designed a type of bipolar probe that is comprised of a hydrophilic DNA part and a hydrophobic conjugated polymer (CP) unit. In aqueous solution, they can form micelles with a hydrophobic CP core and a hydrophilic DNA shell. The aggregation-caused quenching suppresses the fluorescence of CP. Adding telomerase, the hydropathical profile of the bipolar probes is drastically regulated that results in the collapse of micelles and liberates fluorescence simultaneously. The probe has been used in both mimic systems and real urine samples (38 samples). We achieve sensitive and specific detection of telomerase and obtain clearly classification for normal people and cancer patients. It can also be used in a signal off sensor that is used to detect mercury ions